Aqueous Polyphenol Semi-Purified Fractions from Galls of Quercus infectoria Increase ALP Activity and Mineralisation of hFOB 1.19 Cells

The galls of Quercus infectoria (QI) have been reported to possess numerous medicinal values and give a positive effect on bone metabolism. This study investigated the effects semi-purified fractions QI gall extract proliferation, alkaline phosphatase (ALP) activity, mineralisation human foetal osteoblast cell line (hFOB1.19). (fraction A B) were prepared by column chromatography methods. MTT assay was used measure proliferation activity obtain half maximal concentration (EC50) cells treated with fraction (phenolic components contained amide), B presence alkene), pamidronate (drug control). most potent or lowest EC50 further ALP in untreated at day 1, 3, 7, 10, 14 ELISA. Cell determined von Kossa staining for phosphate depositions Alizarin Red S calcium depositions. 8.86 9.92 μg/mL, respectively, which showed greater compared (15.27 μg/mL). ALPactivity both also two control groups (cells cells), starting from 3 onwards. appeared as red spots, while black spots. Interestingly, higher than those groups. In conclusion, enhanced improved mineralisation, increased hFOB 1.19 cells.